indirect antiglobulin test

Methods The blood samples of 23 patients with AIHA were identified by routine typing and typing after chloroquine elution test on DIANA auto instrument. Direct and indirect antiglobulin test were done in the same time.

方法用DIANA全自动血型仪对23例AIHA患者进行ABO、Rh（D）血型常规定型及氯喹放散实验后定型，同时进行直、间接抗人球蛋白试验。

The results showed that out of 38 patients with AIHA, 11 cases ( 31.6%) of ABO blood group were difficultly typed, indirect antiglobulin test were positive, and contradiction between cells typing and sera typing were observed.

结果表明：38例AIHA患者中，ABO血型难定者11例（31.6%），均为间接抗球蛋白试验阳性及正反定型不合。

Result: Using the routine blood group and serology techniques, autoantibody interfered with ABO typing in 10 cases, most of whom were indirect antiglobulin test positive.

结果：28例中10例ABO定型受干扰，多为间接抗球蛋白试验阳性者及正反定型不合者。

The positive rates of direct antiglobulin test ( DAT), elution test and indirect antiglobulin test ( IAT) were 39.2%, 88% and 69.6% respectively.

三项试验中直接抗人球蛋白试验阳性49例（39.2%），释放试验阳性110例（88%），游离试验阳性87例（69.6%）；

Methods DAT positive samples can be divided into AIHA group and non AIHA group, process immunity types, and test the samples of IgG or IgG+ C 3 with elution, use elution liquid and standard antibody screen cell to process indirect antiglobulin test ( IAT).

方法DAT阳性标本分AIHA组和非AIHA组，进行免疫分型，然后把IgG或IgG+C3的标本进行放散试验，利用放散液与标准抗体筛查细胞进行间接抗人球蛋白试验（IAT）。